RESERVES LIST
For lab reports on Lab 4: Bacterial Conjugation
Also look for sources on bacteria, microbiology, molecular biology, genetics, DNA, and biochemistry for information.
Bacteria/Techniques/Indicator Plates/Selection
- 22. Prescott et al., 1999. Microbial Nutrition & Culture Media. (intermediate, from a Microbiology textbook)
- 14. Birge, 1992. Selective and differential media (introductory, from a microbiology textbook).
- 15. Miller, 1972. Behavior of mutants on indicator plates (intermediate, from a laboratory guide)
- 1. Miller, 1972 Dilutions, Plating, and Growth of Bacteria (intermediate, from a laboratory guide)
- 28. QIAGEN, 2001. Appendix A/B: Background Information and Gel Analysis (information about growth, inoculation and use of antibiotics for those using the mini-prep kit from the handbook from our QIAprep kit)
Conjugation and F Factors
- 24. Prescott et al., 1999. Recombination and Plasmids (intermediate, Microbial Genetics chapter from a Microbiology Textbook)
- 25. Black, 1999. Conjugation (intermediate, introduction to subject from a Microbiology textbook).
- 2. Freifelder, 1983. Mechanisms for Exchange of genetic material (intermediate/advanced, from a Molecular Biology textbook)
- 3. Davis et al., 1990. Gene Variation and Transfer (intermediate/advanced, from a Microbiology textbook)
- 4. Miller, 1972. Matings between male and female cells (intermediate, from a laboratory guide)
- 5. Willetts & Skurray, 1987. Structure and Function of the F Factor and Mechanism of congugation (advanced, review chapter from
a book on E. coli)
Plasmids
- 23. Hartwell et al., 2000. Plasmids and Gene Transfer (intermediate)
- 6. Alberts et al.,1989. Viruses, Plasmids, and Transposable Genetic Elements.(intermediate/advanced, from the Advanced Cells (2nd Ed) class textook).
- 8. Alberts et al.,1994. Viruses, Plasmids, and Transposable Genetic Elements.(intermediate/advanced, from the Advanced Cells (3rd Ed) class textook).
- 7. Brock et al., 1994. Genetic Elements: Chromosomes, Viruses, Plasmids (intermediate, from a Microbiology textbook)
- 9. Brock et al., 1994. Plasmids as Cloning Vectors (intermediate, from a Microbiology textbook)
Antibiotics/Resistance Genes
- 26. Tortora et al., 2001. Antimicrobial Drugs (introductory, from a Microbiology textbook)
- 20. Davies, 1994. Antibiotics and Resistance Genes. Review article from the
journal Science: "Inactivation of Antibiotics and the Dissemination
of Resistance Genes". Talks about how antibiotics are inactivated and how
resistance genes move from bacterium to bacterium in nature (intermediate to advanced).
- 11. Alberts et al.,1989. Antibiotics that inhibit protein or RNA synthesis. (summary table and short intro from Advanced Cells textbook).
- 12. Brock et al., 1994. Antibiotics (introductory, from a Microbiology textbook)
- 13. Brock et al., 1994. Antibiotic Resistance in Medicine (introductory, from a microbiology textbook).
Agarose Gel Electrophoresis and Plasmid Isolation
- 27. QIAGEN, 2001. QIAprep MiniPrep Handbook (handbook from our kit)
- 16. Ausubel et al., 1987. Agarose Gel Electrophoresis (from a laboratory guide for workers in molecular biology)
- 17. Ausubel et al., 1987. Mini-preps of plasmid DNA (from a laboratory guide for workers in molecular biology)
- 18. Freifelder, 1983. Methods used to study macromolecules (intermediate/advanced from a Molecular Biology textbook)
Lac Operon
- 19. Miller, 1972 Intro to the lac system (intermediate, from a laboratory guide for experiments with lac+ and lac- strains.
- 21. Hartwell et al., 2000. Gene Regulation in Prokaryotes, including
the lac operon (intermediate, from a Genetics textbook)
CITATIONS
A few of reserves are from the following sources:
- Ausubel, F. M. and others, ed. Current Protocols in Molecular Biology (John Wiley & Sons, NY). (For your class lab report, you can abbreviate the citation like this, the list of editors is very long and it is in 3 volumes).
- Miller, Jeffrey., 1972. Experiments in Molecular Biology. Cold Spring Harbor Laboratory (Cold Spring Harbor, NY) 466p. -very relevant material for our conjugation experiment
- If you cite information from several of the reserve articles but these came from a single book, you can use a footnote in your citations list to tell which articles so that your instructor knows that you did indeed consult more than one "article".
GENETICS - TEXTBOOKS
Books for overnight check-out. PLEASE DO NOT WRITE IN THEM
these will stay on reserve throughout the semester
Introductory GENETICS textbooks (intermediate)
- Russell, Peter J. 1992. Genetics (2nd edition). This is the LARGER VERSION of
the genetics textbook written by Russell, your class text is the reduced version.
- Brooker, Robert J. 2005. Genetics: Analysis and Principles, 2nd Edition (hardback)
- Klug, W.S., M. R. Cummings and C.A. Spenser2006. Concepts of Genetics (8th Ed.) hardback edition
Introductory BIOLOGY textbooks :
- Raven & Johnson. 1996. Biology 4th Edition (1310 pages, hardcover)
- Starr, Cecie.1991. Biology: Concepts & Applications (599 pages, hardcover)
- Curtis & Barnes. 1989 Biology (5th Ed.). Part I:Biology of Cells (403 pages, softcover)
To return to theBIO 1015 Genetics home page
Last revision:4/11/08
Current Address: http://www.sju.edu/biology/dratterm/rconj.htm
The SJU Biology Department home page can be accessed at the short address: http://www.sju.edu/biology