Complementation of a Ustilago maydis Temperature Sensitive Mutant

6/29/01

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Table of Contents

Complementation of a Ustilago maydis Temperature Sensitive Mutant

What is a TS mutant ?

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Making TS Mutants

Phenotype of Mutant 28-44

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Phenotype of Mutant 28-44

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Complementation

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Complementation

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Subcloning

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Step 1- Restriction Enzymes

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Step 1- Restriction Enzymes

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Step 2- Ligations

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Step 3- Transformations

Step 4- Repeat

The Last Subclones

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After Subcloning -Future Plans

Acknowledgements

 Author: Jen Johnson 

Home Page: http://www.sju.edu/biology/ksweb/ustilago/intro.html