Biological Safety Manual
1. Definition of Biohazards and Potentially Infectious Material
2. Procedures for Biohazard Control
3. Class II Biological Safety Cabinets
4. Posting of Biological Hazard Signs
a. Biohazard Spills inside a Biological Safety Cabinet
b. Biohazard Spills outside a Biological Safety Cabinet
c. Large Spill of Biosafety Level 2 Material (>500ml)
d. Personal Contamination
e. Decontamination and Clean Up of Blood Spills
6. Biological Risk Assessments
a. Quick Guide to Risk Assessment for Biological Hazards in the Laboratory
a. Definitions
9. Procedures for the Safe Disposal of Biohazardous Waste
10. Agents required to be Autoclaved or Decontaminated Prior to Disposal
11. Decontamination, Sterilization and Disinfection
a. General
b. Definitions
c. Methods
12. Activity Levels of Selected Liquid Germicides
13. Shipping of Biological Materials and Dry Ice
a. Table 1: Category A Infectious Substances
b. Table 2: Shipping Summary Table
c. Packing Instructions 620 Summary
d. Packing Instructions 650 Summary
e. Packing Instructions 954 Summary
f. Packing Instructions 959 Summary
g. Exempt Packing Instructions
h. Permits
15. Research Involving Recombinant and Synthetic Nucleic Acid Molecules
a. IBC Procedures
b. IBC By-laws
c. IBC Meeting Schedule
16. CRISPR/Cas9 Biosafety Guide
17. Institutional Biosafety Committee Information
19. Summary of Recommended Biosafety Levels for Infectious Agents and Infected Animals
a. Table 1 - Summary of Recommended Biosafety Levels for Infectious Agents
b. Table II - Summary of Recommended Biosafety Levels for activities in which experimentally or naturally infected vertebrate animals are used
20. Laboratory Practices for each Biosafety Level and Biosafety Level Criteria
21. Vertebrate Animal Biosafety Level Criteria for Vivarium Research Facilities
22. Biosafety Level 2+ (Enhanced)
23. Classification of Human Etiologic Agents on the Basis of Hazard (Appendix B)
a. Oncogenic Viruses, Bacteria, Fungal Agents, Parasitic Agents, Viruses and Prions (See Appendix B of the NIH Recombinant or Synthetic Nucleic Acid Guidelines)
24. Laboratories Working with HIV and Hepatitis Viruses
25. Standard Operating Procedure for Handling Animals Dosed with Toxic Chemicals
26. Standard Operating Procedure for Biological Toxins
27. Standard Operating Procedure for Biosafety Level 2
28. Diseases Communicable From Animals to Humans
29. Recommended Procedures for Preventing the Production of Aerosols
a. General
b. Centrifuge Operation
c. Mixing Operations
d. Vacuum and Aspirating Equipment
e. Needles and Syringes
f. Pipettes
g. Transfer Loops
30. Recommended Procedures for the Proper Use of Biological Safety Cabinets
31. Biological Safety Cabinets and the Risks Using Open Flames