Laboratory Safety Manual (Chemical Hygiene Plan)

1. Introduction

2. Scope

3. Responsibilities

4. Raising Safety Concerns

1. Chemical Hygiene and Safety Plan

    a. General Standard Operating Procedures

2. Laboratory Housekeeping

3. Training Requirements

4. Hazardous Chemicals

5. Warning Labels

6. Safety Data Sheets

7. Toxicology Overview

    a. Chemical Toxicity

    b. Dose Response Relationship

    c. Route of Entry

    d. Health Effects

    e. Other Factors Affecting Toxicity

8. Prior Approval

9. Research Protocol Form

10. Working After-Hours/Alone in Laboratories

11. Unattended Experiments

12. Laboratory Security

13. Minors/Volunteers in Laboratories

1. Personal Protective Equipment and Clothing

    a. General Policy

    b. Eye and Face Protection

    c. Skin Protection and Lab Coats

    d. Respirators

    e. Personal Protective Equipment (PPE) Hazard Assessment

    f. Selection Chart for Eye and Face Protection

2. Laboratory Fume Hoods

1. Fire Safety and Evacuation Procedures

2. Chemical Spills

3. Personal Contamination

    a. General Information

    b. Chemicals Spilled on the Skin

    c. Chemicals in the Eyes

4. Special Procedures for Hazardous Substance Exposures and Incidents

    a. Reporting Hazards

    b. Hazardous Substance Exposure

    c. Employee Hazardous Substance Exposure/Accidents

    d. Student Hazardous Substance Exposure/Accidents

    e. Reporting of "Near Miss" Accidents

1. Purchases, Donations and Transfers of Hazardous Materials

2. Purchase, Use and Disposal of Controlled Substances and Precursors

3. Chemical Storage

    a. General Considerations for Chemical Storage

    b. Flammable and Combustible Liquid Storage

    c. Chemical Stability

    d. Shock Sensitive Chemicals

    e. Compounds that form Peroxides during Storage

    f. Compressed Gas Storage

4. Transportation of Hazardous Chemicals On-Campus

5. Shipping and Permitting of Biological Materials and Dry Ice

1. Waste Disposal

    a. Introduction

    b. Responsibilities

    c. Packaging and Labeling Procedures

    d. Waste Reduction

    e. Definitions

2. Disposal Procedures for Electrophoresis Buffer Solutions and Gels

3. EHRS's Chemical/Hazardous Waste Checklist for EPA Compliance

4. EPA's (Environmental Protection Agency) Hazardous Waste List

5. Laboratory Relocation/Clean-Out Procedures

1. Safe Handling of Flammables

2. Safe Handling of Reactive and Peroxide Forming Chemicals

3. Safe Handling of Corrosive Chemicals

4. Safe Handling of Compressed Gases

5. Safe Handling of Nanomaterials

6. Safe Handling of Glassware

7. Quick Guide to Risk Assessment for Toxicity, Physical, Flammable, Explosive and Reactive Hazards in the Laboratory

8. Standard Operating Procedures for Obtaining Safety Data Sheets

9. Standard Operating Procedures for Reproductive Hazards, Carcinogens, or Acutely Toxic Chemicals

10. Standard Operating Procedures for Hydrofluoric Acid

11. Standard Operating Procedures for Pyrophoric Chemicals

12. Standard Operating Procedures for Piranha Solutions

13. Standard Operating Procedures for Gel Electrophoresis

14. Standard Operating Procedures for Sodium Azide

15. Standard Operating Procedures for Cytotoxic Agents

16. Standard Operating Procedure for Water Sensitive Chemicals

17. Standard Operating Procedures for Biological Toxins

18. Standard Operating Procedure for Biosafety Level 2

19. Standard Operating Procedure for Handling Animals Dosed with Toxic Chemicals

20. Standard Operating Procedure for Liquid Nitrogen and Cryovial Safety

21. Diseases Communicable From Animals to Humans

22. 3D Printing Safety

23. Recommended Procedures for Preventing the Production of Aerosols

24. Recommended Procedures for the Proper Use of Biological Safety Cabinets

25. Recommended Procedures for Autoclaving Materials

26. Recommended Procedures for Vacuum Equipment and Vacuum Pumps

27. Oxygen Deficiencies in Cold Rooms and Warm Rooms

28. The National Academies "Prudent Practices in the Laboratory"

29. American Chemical Society "Safety in Academic Chemistry Laboratories" (8th Edition)

30. American Chemical Society "Guide for Chemical Spill Response Planning in Laboratories"

31. Waste Anesthetic Gases (WAG) - Animal Research

1.List of Some Carcinogens and Suspect Carcinogens

2. List of Some Reproductive Hazards

3. List of Some Acutely Toxic Chemicals

4. Examples of Incompatible Chemicals

5. Compounds That Form Peroxide During Storage